Use of diluted glycerine and 70% alcohol as inert vehicles in the isotherapics from pure bacterial cultures. preparation of
Tests were made to verify the viability of using diluted glycerin and alcohol 70% (p/p) as inert substances in the preparation of isotherapics, starting from pure bacterial cultures of Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas aeruginosa, Staphylococcus aureus e Streptococcus beta hemolytic of A Group. The isotherapics were prepared by Hahnemannian method until 12 CH, starting from suspensions of the lineage in diluted glycerin, with standardized turbidity (Grade 3 on Mac Farland Scale). The first three dynamizations were prepared using the same inert substance of the starting suspension. In the following dynamization alcohol 70% (p/p) was used.After that the isotherapics were submitted to verification tests of diluted glycerin and alcohol 70% (p/p) bactericide action over the dynamized microorganism, using the technique of quantitative sowing.In all the samples, the following results were observed:a) there was growing of uncountable colonies in the first two dynamizations; b) in the third dynamization this growing reached indexes close to 3,000 UFC/ml; c) starting from 4 CH it was not observed bacterial growing. The bacterial growing in the first three dynamizations reveals the innocuity of diluted glycerin to the dynamized lineage.On the other hand, the non-growing from 4 CH shows the antibacterial action of alcohol 70% (p/p). The use of diluted glycerin and alcohol 70% (p/p) in the preparation routines of isotherapics must be prioritized to other inert substances unreported on Brazilian Homeopathic Pharmacopoeia.