Pilot study on the action of Nux vomica 12cH os mice subjected to sleep deprovation.

  • Karina F. L. R. de O. Figueiredo FACIS e Centro de Ensino Superior de Homeopatia-IBEHE
  • Leoni V. Bonamin Instituto de Ciéncias da Saúde,Laboratório de Patologia Veterinária-Universidade de Santo Amaro.
  • Luiselle P. Miranda FACIS e Centro de Ensino Superior de Homeopatia-IBEHE
  • Vânia D' Almeida Laboratório de Erros Inatos de Metabolismo-Universidade Federal de São Paulo
Keywords: Homeopathy, sleep deprivation, Nux vomica, anxiolytic effect, mice


This work was intended to evaluate the effects of the homeopathic medicine Nux vomica 12CH in stressed mice submitted to a sleep deprivation protocol. The remedy was chosen according to the homeopathic similarity law. Male mice were divided in 4 groups: sleep deprived (SD); animals allowed to sleep (control) and treated with water; SD treated with Nux vomica 12 CH (SD-NV); and control mice treated with Nux vomica (NV). The animals were exposed to sleep deprivation for 3 days. The animals were weighed and submitted to open field (OF) and elevated plus maze (PM) tests before and after the SD. At the end of the experiment, the animals were sacrificed.During the experiment, 10 µl of water or medicine were given orally to the mice, daily, between 9 and 10 a.m. The experiment was blindly conducted. All sleep deprived animals had decrease of weight gain, independent of the treatment employed. The group of animals treated with NV produced more excrement on second OF exposition, but the results, taken together, suggest that Nux vomica 12CH had no anxiolytic effects on sleep deprived animals.

Basic Research (Biology)