In vitro behavior of Mycoplasmagallisepticum live-type nosode

  • Môsar Lemos Universidade Federal Fluminense (UFF)
  • Elmiro Rosendo do Nascimento Departamento de Medicina Coletiva Veterinária e Saúde Pública, Faculdade de Veterinária, UFF
  • Maria Lucia Barreto Núcleo de Animais de Laboratório, UFF
  • Virginia Leo de Almeida Pereira Departamento de Medicina Coletiva Veterinária e Saúde Pública, Faculdade de Veterinária, UFF
  • Cátia Cardoso da Silva Núcleo de Animais de Laboratório, UFF, bolsista FAPERJ
  • Jenif Braga de Souza Centro de Experimentação Animal, IOC/FIOCRUZ
  • Sabrina da Silva Barros Centro de Experimentação Animal, IOC/FIOCRUZ
  • Leandro dos Santos Machado Programa de Pós-Graduação em Higiene Veterinária e Processamento Tecnológico de Produtos de Origem Animal, Faculdade de Veterinária, UFF
  • Carlos Augusto de Martino Campos Núcleo de Animais de Laboratório, UFF
Keywords: Biotherapy, Chickens, Live nosode, Mycoplasmagallisepticum

Abstract

As a step of a doctoral research project, in this study a live-type nosode was prepared from microorganism Mycoplasmagallisepticum strain R (ATCC 93-08/19610) according to Costa model and the rules by Brazilian Homeopathic Pharmacopoeia. Live nosode was tested in vitro to assess safety when used to immunize domestic fowl (Gallus gallus) against infection by this microorganism and to investigate its behavior under laboratory conditions. M. gallisepticum was not shown to grow in fluid (broth) and solid (plate) modified Frey medium with dilutions 11d, 12d, 20d and 30d. Inhibition halos about 2.0 mm were observed around paper disks impregnated with live-type nosode in microorganism-sown Petri dishes, whereas disks impregnated with conventional antibiotic oxytetracycline exhibited 8.0 mm inhibition halos. Protein assessment by Folin-Lowry method showed protein absence in dilutions 12d and 30d and neither microbial DNA traces were found in PCR assay in dilutions 12d, 20d and 30d.
Published
2011-12-09
Section
Veterinary Research