Causticum hahnemanni, Conium maculatum and Lycopodium clavatum highly diluted medications decreases parasitemia in mice infected by Trypanosoma cruzi

  • Gislaine Janaina Sanchez Falkow Temporini Universidade Estadual de Maringá
  • Carina Ribeiro Lopes Universidade Estadual de Maringá
  • Camila Fernada Brustolin Universidade Estadual de Maringá
  • Paula Fernanda Massini Universidade Estadual de Maringá
  • Érika Cristina Ferreira Universidade Estadual de Maringá
  • Franciele Karina da Veiga Universidade Estadual de Maringá
  • Denise Lessa Aleixo Universidade Estadual de Maringá
  • Nelson Roberto Pala Homeopata voluntário
  • Terezinha Barion Homeopata Voluntária
  • Luiz Gilson Esper Veterinário Homeopata Voluntário
  • Silvana Marques de Araujo Universidade Estadual de Maringá
Keywords: Chagas’ disease, High dilutions, Mice, Trypanosoma cruzi

Abstract

Introduction: Benznidazole is the only medicine available in Brazil for Chagas’ disease treatment, however it presents low efficacy in the chronic phase and several adverse effects [1-3]. Aim: Evaluate the effect of Causticum hahnemanni, Conium maculatum and Lycopodium clavatum [4-6] administered to mice infected with T. cruzi. Method: In blind randomized controlled trial 42 male Swiss mice, 8 weeks of age, have been grouped: GCaus –treated with C. hahnemanni 13cH (n=10), GCon –treated with C. maculatum 13 cH (n=11), GLy –treated with L. clavatum13cH (n=10) and CG – control group (n=11) treated with 7% hydro alcoholic solution 13cH. The animals were infected intraperitoneally with 1.400 blood trypomastigotes of T. cruzi - Y strain. Medications were been prepared according to Brazilian Homeopathic Pharmacopoeia [7]. Medication was diluted in water (1mL/100mL) offered ad libitum, from amber recipient during 16 hours administered 48 hours before infection and 48, 96 and 144 hours after inoculation. Parasitological parameters assessed: total parasitemia (TP), maximum peak of parasites (MPP), pre-patent period (PPP) and area under curve (AUC). Parasitemia was evaluated daily counting from the first day of infection. Clinical parameters assessed: weight, temperature, water and food intake and excreta were measured counting from the 5º day before infection until animals’ death or checking negative parasitemia for 3 consecutive days. Mortality was registered for 75 days after infection. Ethics Committee for Experiments in Animals gave approval UEM 054/11. Statistical comparison of data was performed with Kruskal-Wallis test, with 5% significance. Results and Discussion: The diluted medications have been significantly reduced the parasitological parameters: MPP (p<0,0000), TP (p<0,0000) and AUC (9,7±3,5)x109; (6,2±1,7)x109; (5,5±1,7)x109; (5,7±1,5)x109 (p<0,0001) measured in trypomastigotes/mL, considering CG, GCaus, GCon and GLy respectively. Besides, they increased the PPP for GCaus, GCon and GLy (5,07±0,54; 5,40±1,29; 5,91±1,1) in relation to CG (4,75±1,00) (p<0,0001). Survival was significantly different between groups (p=0,0001), with Ly showing survival estimate of 0,29 (IC: 0,182 – 1) versus 0,125 from CG (IC: 0,02 – 0,782) until the 21st day of infection. GCaus and GCon showed survival estimate 0 for 18 and 17 days, respectively. GLy presented significant increase in water intake (p=0,0000) and higher temperature control, lowering hypothermia before death (p<0,0000). Conclusion: The high diluted medicines evaluated showed different performances. Lycopodium clavatum showed the best benefits for animals infected with lower parasitemia, best clinical development and greater survival.

Author Biographies

Gislaine Janaina Sanchez Falkow Temporini, Universidade Estadual de Maringá
Departamento de Ciéncias Básicas da SaúdeParasitologia - Laboratório de Doença de Chagas
Carina Ribeiro Lopes, Universidade Estadual de Maringá
Departamento de Ciéncias Básicas da SaúdeParasitologia - Laboratório de Doença de Chagas
Camila Fernada Brustolin, Universidade Estadual de Maringá
Departamento de Ciéncias Básicas da SaúdeParasitologia - Laboratório de Doença de Chagas
Paula Fernanda Massini, Universidade Estadual de Maringá
Departamento de Ciéncias Básicas da SaúdeParasitologia - Laboratório de Doença de Chagas
Érika Cristina Ferreira, Universidade Estadual de Maringá
Departamento de Ciéncias Básicas da SaúdeParasitologia - Laboratório de Doença de Chagas
Franciele Karina da Veiga, Universidade Estadual de Maringá
Departamento de Ciéncias Básicas da SaúdeParasitologia - Laboratório de Doença de Chagas
Denise Lessa Aleixo, Universidade Estadual de Maringá
Departamento de Ciéncias Básicas da SaúdeParasitologia - Laboratório de Doença de Chagas
Silvana Marques de Araujo, Universidade Estadual de Maringá
Departamento de Ciéncias Básicas da SaúdeParasitologia - Laboratório de Doença de Chagas
Published
2012-08-10