Antimalarial potential of homeopathic medicines against schizont maturation of Plasmodium berghei in short-term in vitro culture

  • Aswathy Rajan Department of Zoology, PANJAB UNIVERSITY, CHANDIGARH, INDIA
  • Upma Bagai Department of Zoology, Panjab University, Chandigarh
Keywords: Cinchona officinalis, Chelidonium majus, Arsenicum album, Homeopathy, Plasmodium berghei

Abstract

In vitro assessment of antimalarial drug susceptibility of Plasmodium has been a major research success, which has paved the way for the understanding of parasite and rapid screening of antimalarial drugs for their effectiveness. In the present study a preliminary screening to check the antiplasmodial activity of mother tincture (ϕ) and various potencies (6C, 30C, 200C) of homeopathic medicines Cinchona officinalis/china (Chin.), Chelidonium majus (Chel.) and Arsenicum album (Ars.) were done by assessing the in vitro schizont maturation inhibition assay. A significant reduction in the growth of intraerythrocytic stages of P. berghei was observed with decreasing dilution of ϕ and various potencies of Chin., Chel. and Ars. exhibiting a dose dependent effect. Maximum schizont maturation inhibition was observed by Chin. ϕ (1:1), Chin. 30 (1:1, 1:2) and Chel. 30 (1:1) i.e. 80%. The standard drug CQ at 10 µM concentration exhibited 95.4±1.6% inhibition of schizont maturation. Ars. 30 (1:1) also have been found to possess strong antiplasmodial efficacy with 75.5±2.6% schizont inhibition. The presence of free merozoites in Ars. 200 with weak schizonticidal inhibition activity (40-45%) also pointed towards the ability of parasite to survive in the given drug pressure.

Author Biographies

Aswathy Rajan, Department of Zoology, PANJAB UNIVERSITY, CHANDIGARH, INDIA
Department of Zoology Panjab University Chandigarh India
Upma Bagai, Department of Zoology, Panjab University, Chandigarh
Department of Zoology Associate Professor
Published
2012-12-19
Section
Basic Research (Biology)