Experimental protocol: Atropa belladonna highly diluted for the treatment of Trypanosoma cruzi infection
Keywords: Atropa belladonna, Chagas' disease, Mice, Trypanosoma cruzi.
AbstractBackground: Natural products are a potential source of new drugs which may present fewer side effects . The analysis of Atropa belladonna in different experimental models is suggested as object of study considering the Multicenter International Project on High Dilution. Aim: To evaluate the effect of highly diluted medicine of Atropa belladonna under murine infection by T. cruzi. Methodology: Project approved by the Animal Ethics Committee UEM. The experiments will be done under blind, controlled and randomized testing by draw. One hundred and twenty swiss male, mice, 8 weeks old, will be divided into groups with 12 animals according to the treatment: CI - infected non treated; CNI- non infected and non treated; infected and treated animals 48 hours before and after infection, subsequently were treated 56/56 hours until 9th day of infection with TM - A. belladonna mother tincture and in the 2cH; 3cH; 5cH; 6cH; 12cH e 30cH dilutions. Animals will be infected with 1400 blood trypomastigotes of T. cruzi, strain Y, via ip. Medicines are going to be prepared according to the Brazilian Homeophathic Pharmacopoea  and offered diluted in water ad libitum, in an amber drinker during 16 hours. Parasitological parameters evaluated: total parasitemia, total peak of parasites, pre-patent period and area under the curve. The parasitemia will be evaluated daily from the 1st day of infection until negative findings for 3 consecutive days. Histological parameters of spleen, heart, liver, intestine, skeletal muscle, and immunohistochemical analysis of the apoptosis process. Statistical comparisons will be analyzed by the Software Statistic 8.0 with 5% of significance. Considerations: Since the Multicenter International Project on High dilution intends to study the same medicine in several experimental models and, moreover, the murine model of infection by T. cruzi is widely known, it will be possible to suggest probable mechanisms of action to the tested medicines.