Effect of medication produced with cysts of Toxoplasma gondii in mice infected with this protozoan

  • Caroline Felicio Braga
  • Denise Lessa Aleixo
  • Neide Martins Moreira
  • Ricardo Nascimento Drozino
  • Franciele Karina da Veiga de Sales
  • Carina Ribeiro Lopes
  • Camila Fernanda Brustolin
  • Paula Fernanda Massini
  • Silvana Marques de Araújo
Keywords: Toxoplasmosis, Toxoplasma gondii, biotherapy, TGF-beta.


Under test blind, controlled, randomized by draw, the effect of different potencies of T. gondii biotherapic were compared. Fifty-six male mice, Swiss, 60 days-old, were divided into groups according to the treatment: BIOT-TG7, BIOT-TG17, BIOT-TG30, TG60-BIOT, BIOT-TG100, TG200-BIOT, GCInf-infected control group treated with grain alcohol and BCM-7% - non treated and non infected. The biotherapics were produced according to the Brazilian Homeopathic Pharmacopoeia, with macerated mouse brain (20 cysts T. gondii/100μL). The animals were treated for three consecutive days prior to infection. Groups BIOT-TG7, BIOT-TG17, BIOT-TG30 and BIOT-TG60 and GCInf 0.1mL/4X/day received on the first day and 2X/day on the other days of treatment. For BIOT -TG100 and BIOT- TG200 were used 0.1mL/single dose / day. After 60 days the animals were infected (ME49 strain 20-T gondii cysts), orally. Fundoscopy and ocular tononetry were conduced at 55 days post-infection. Sixty days after infection the number of cysts were counted and serum TGF-β (ELISA) were dosed . For statistical comparison were used the Kruskal-wallis test, 5% statistical significance and Effect Size Measures. In tonometry, there was no significant difference between the control group and the other groups. Regarding to ocular fundoscopy, it was found that 80% of group BIOT-TG100 did not present amendments and 20% presented mild subretinal hemorrhage surrounding the optic nerve differently from the other groups. In BIOT-TG200 group, 50% of the animals presented mild hemorrhage and 50% remained unchanged. The BIOT-TG200 presented a decrease in the number of cysts in the brain. There was no difference observed between the BIOT-TG100 and the control group. Animal group BIOT-TG100 presented higher (p <0.01) concentrations of TGF-ï¢ in comparison to BIOT-TG200 group. The highest dilutions BIOT-TG100 and BIOT-TG200 provided more effective benefits, although the levels of TGF-ï¢ have changed in opposite ways in the two treatments. This result deserves further studies.