Extremely Dilution of Strychnos Nux vomica Mitigates Alcohol-Induced Reduction in Enthalpies Associated With Free Water Molecules in a Fish Brain

  • Atheni Konar M.Sc
  • Tandra Sarkar M.Sc
  • Nirmal Chandra Sukul
  • Md. Amir Sohel
  • Abhijit Mondal
  • Asmita Sengupta

Abstract

Introduction
Alcohol intoxication affects aquaporins in the glial cells of brain resulting in oedema. Nux vomica, a homeopathic drug of plant origin, is known to counteract alcohol effect. The objective of this present study is to find out the level of free water molecules in the brain of a teleost fish under ethanol intoxication. The second objective is to determine whether Nux vomica could restore the level of free water in the alcohol treated fish.
Materials and methods
One group of fish was exposed to 456 mM ethanol for 30 min, another exposed first to a solution of Nux vomica 200c for 20 min and then to 456 mM ethanol for 30 min. The third group served as an untreated control. The mid brain of each fish was kept in an aluminium sample pan and its free water level was assessed by differential scanning calorimetry (DSC).
Results
All alcohol treated fish showed significant reduction in the level of free water molecules as compared to the untreated control. Treatment with Nux vomica increased the level of free water in the brain significantly as compared to the untreated alcoholic group.
Conclusion
Alcohol intoxication reduces free water molecules in the fish brain. Nux vom might have acted on aquaporins in the glial cells thereby increasing the level of free water in the brain.

Author Biographies

Atheni Konar, M.Sc
Centre for Healthcare Science and Technology, IIEST (Indian Institute of Engineering, Science and Technology)
Tandra Sarkar, M.Sc
Centre for Healthcare Science and Technology, IIEST (Indian Institute of Engineering, Science and Technology)
Published
2018-12-03
How to Cite
KONAR, Atheni et al. Extremely Dilution of Strychnos Nux vomica Mitigates Alcohol-Induced Reduction in Enthalpies Associated With Free Water Molecules in a Fish Brain. International Journal of High Dilution Research - ISSN 1982-6206, [S.l.], v. 17, n. 3-4, p. 2, dec. 2018. ISSN 1982-6206. Available at: <http://highdilution.org/index.php/ijhdr/article/view/908>. Date accessed: 19 dec. 2018.
Section
Basic Research (Biology)