Physical transfer of the UHD signal resulting from potentized dilution of interferon gamma antibodies


  • Igor Jerman BION Institute, Stegne 21, Ljubljana, Slovenia, EU
  • Linda Ogrizek
  • Luka Jan
  • Vesna Periček



Physico-chemical examinations of very high dilution (UHD) solutions subjected to certain physical factors (such as shaking) are becoming more frequent and are increasingly producing conclusive results. A much less studied phenomenon is the transfer of molecular information (UHD signals of diluted substances) from one liquid to another without an intermediate liquid phase. The aim of this study was to investigate the possibility of such a transfer of the UHD signal from the UHD solutions to the receiver solution, in particular, if the molecular source used in the donor solutions was the biologically active interferon gamma antigen molecule. We were especially interested in how the transfer of the UHD signal is affected by the time of exposure of the receiver to the donor, and how the transfer is affected by activation (shaking) versus exposure alone.

For measurement of the physicochemical parameters, we used pH, electrical conductivity, and oxidation-reduction potential (ORP) sensors. For relative UV/VIS spectroscopy measurements, we used a Macherey-Nagel spectrophotometer. All original liquids (water and solutions, four different liquids altogether) subject to research were obtained from Materia Medica LTD, Moscow. Distilled water (W) was used for dilutions and potencies as follows: potentized water (W cH9), potentized dilution of antibodies to interferon-gamma (Abs IFNγ cH9), the same original substance in the form of a mixture of potencies (Abs IFNγ cH12, cH30, cH50, shortly Abs IFNγ mix). We used two different receiver solutions, one for physicochemical research and another (addition of alcohol) for UV/VIS spectroscopy. The transfer of UHD signal via glass was processed via shaking of the donor flask. For direct or post-hoc analysis, we used Wilcoxon signed-rank test, two tailed. The results showed that activation strongly influences signal transfer and that this can be compensated to some extent by prolonged direct exposure. In principle, exposure time has a positive effect on signal transfer. Although the detection of the physically transmitted signal is generally weaker than the signal in the original UHD solutions, it is still detectable with our measurement system and protocol, both in terms of effect (Cohen's D) and statistically significant differences. In this respect, different detection methods may detect the signal differently. After the separation between the donor and receiver glasses (solutions), the character of the signal may change and may even be better expressed. Activation is shown to be an important factor for the UHD signal transfer, at least for shorter times. By longer exposure times, it appears that activation can be compensated, at least to some extent.




How to Cite

Jerman, I., Ogrizek, L., Jan, L., & Periček, V. (2023). Physical transfer of the UHD signal resulting from potentized dilution of interferon gamma antibodies. International Journal of High Dilution Research - ISSN 1982-6206, 22(2), 34–35.

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