Effects of homeopathic preparations of Viscum album on in vivo murine melanoma model

Authors

  • Thais C da Silva 1Experimental and Environmental Pathology, Universidade Paulista – UNIP
  • Carla Quaresma Holandino 2 Pharmacy College, federal University of Rio de Janeiro – UFRJ
  • Sandra A Gordinho Pinto 1Experimental and Environmental Pathology, Universidade Paulista – UNIP
  • Débora de Oliveira Mares Silvestro 2 Pharmacy College, federal University of Rio de Janeiro – UFRJ
  • Ivana Barbosa Suffredini 1Experimental and Environmental Pathology, Universidade Paulista – UNIP
  • Priscila Inhauser Baltuille do Prado 1Experimental and Environmental Pathology, Universidade Paulista – UNIP
  • Hellen Denise Rocha 1Experimental and Environmental Pathology, Universidade Paulista – UNIP
  • Bárbara Hiramuki
  • Vitoria Yasmin Silva Laranja 1Experimental and Environmental Pathology, Universidade Paulista – UNIP
  • Leoni Villano Bonamin 1Experimental and Environmental Pathology, Universidade Paulista – UNIP
  • Elizabeth Cristina Perez 1Experimental and Environmental Pathology, Universidade Paulista – UNIP

DOI:

https://doi.org/10.51910/ijhdr.v22i2.1364

Keywords:

Skin cancer, B16F10 cells, homeopathic potencies, mistletoe, Quercus robur, cancer-related fatigue, integrative therapies

Abstract

Recently, several authors have shown the benefits of homeopathy in improving the quality of life
and survival of cancer patients. Preliminary data from the group showed that B16F10 murine
melanoma cells treated with Viscum album (Va), collected from the host tree Quercus robur during
the Swiss summer (VaQr.s) and prepared in different homeopathic scales: 12 dH, 200 cH and 5 lm
decreased both viability and production of reactive oxygen species (ROS) and MCP-1 cytokine,
when compared to B16F10 cells treated with the chemotherapy drug carboplatin. However, the in
vivo effects of these treatments have not been evaluated. Therefore, the aim of the present work
was to investigate the effects of different homeopathic preparations of VaQr.s in mice with
melanoma. For this, C57BL/6 mice were inoculated subcutaneously (s.c) on the left flank with
B16F10 cells. After appearance of tumor nodule, mice were treated three times a week, orally,
during 3 weeks with mother tincture (TM) or different potencies of VaQr.s (12 dH, 200 cH or
5 lm). As controls, mice were treated with water or vehicle (10%-alcohol) or chemotherapy
(carboplatin). Evaluations of the animals' general condition and tumor growth were performed on
alternate days until approximately 30 days after tumor cell inoculation. The results obtained
showed that mice treated with VaQr 12 dH or 5 lm have increased survival and body weight, as
well as a tendency to increase the quality of life of animals with melanoma, since these presented
less clinical manifestations when compared to the other treatments, mainly the carboplatin group.
Together, the data presented here demonstrated for the first time, the effects of Va potencies on
alleviating cancer-related fatigue in an in vivo model of murine melanoma. Furthermore, this work
provides relevant information for understanding the molecular events induced by homeopathic
preparations of Va as a support for future studies with ultradiluted Va

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Published

2023-12-19

How to Cite

da Silva, T. C., Quaresma Holandino, C., Gordinho Pinto, S. A., de Oliveira Mares Silvestro, D., Barbosa Suffredini, I., Inhauser Baltuille do Prado, P., Rocha, H. D., Hiramuki, B., Silva Laranja, V. Y., Villano Bonamin, L., & Perez, E. C. (2023). Effects of homeopathic preparations of Viscum album on in vivo murine melanoma model. International Journal of High Dilution Research - ISSN 1982-6206, 22(2), 24–24. https://doi.org/10.51910/ijhdr.v22i2.1364

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