Homeopathic dilutions of Phytolacca decandra induce cytotoxicity of human breast adenocarcinoma cells
Keywords:MCF7 cell line, P. decandra, doxorubicin, Hahnemannian dilution, integrative medicine, apoptosis, inflammatory cytokine
Breast cancer is a malignant tumor frequently diagnosed in the female population that represents
the main cause of cancer death in women worldwide. Recently, the use of complementary therapies
such as homeopathy has increased to alleviate the side effects of conventional allopathic treatments.
However, the cellular and molecular effects of homeopathic medicaments have not been fully
clarified. Thus, the aim of this work was to evaluate the in vitro effects of Phytolacca decandra
homeopathic preparations on human breast adenocarcinoma cells. For this, MCF-7 breast
adenocarcinoma cells were cultivated in the presence or absence of Phytolacca decandra mother
tincture (PdTM) or centesimal Hahnemannian dilution (CH) of Phytolacca decandra: 30 or 200 CH.
Determination of apoptosis and necrosis percentages, cytokine production and morphological
changes were evaluated after 24, 48 and 72 hours of culture in each of the treatments. Results
obtained in cytotoxicity analyses showed a higher percentage of death in cells treated with
homeopathic dilutions, with similar results to that presented by cells treated with Doxorubicin or
PdTM. In contrast, cells treated with homeopathic dilutions of Phytolacca decandra did not show
marked differences in the production of pro and anti-inflammatory cytokines when compared to
untreated. Similarly, analyses of cell morphology did not display significant differences before and
after treatment with homeopathic dilutions, when compared to untreated cells. Already, cells treated
with PdTM presented marked cellular alterations with observation of apoptotic bodies in the three
evaluated times. Together, results show that homeopathic dilutions of Phytolacca decandra induced
cytotoxicity of human breast adenocarcinoma cells in the absence of inflammatory process.
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Copyright (c) 2023 Elizabeth C. Perez, Larissa Béja, Thiago Albuquerque Viração, Jaqueline de Carvalho Arjona, Sandra Kalil, Leoni Villano Binamin
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