Physical chemical and citotoxic evaluation of highly diluted solutions of Euphorbia tirucalli L. prepared through the fifty milesimal homeopathic method

Authors

  • Sheila Garcia Faculty of Pharmacy, Federal University of Rio de Janeiro -UFRJ, Rio de Janeiro
  • Rafael Cardoso Harduim Faculty of Pharmacy, Federal University of Rio de Janeiro -UFRJ, Rio de Janeiro
  • Fortune Homsanin Faculty of Pharmacy, Federal University of Rio de Janeiro -UFRJ, Rio de Janeiro
  • Carlos Renato Zacharias UNESP
  • Ricardo Machado Kuster Núcleo de Pesquisa em Produtos Naturais, UFRJ
  • Carla Holandino Quaresma Faculty of Pharmacy, Federal University of Rio de Janeiro -UFRJ, Rio de Janeiro

Keywords:

High dilutions, LM method, electrical conductivity, pH, refraction index, cytotoxicity, Euphorbia tirucalli

Abstract

Background: although Hahnemann described the fifty-milesimal (LM) method in the 6th edition of the Organon of the Medical Art, very little research has been carried out on the physical chemical properties of these homeopathic preparations. Furthermore, there is still no evidence allowing for the correlation between the alleged physical chemical properties and the biological effects of high dilutions. Aims: to evaluate physical chemical characteristics of LM preparations including electrical conductivity, pH and refraction index, and their effect on biological experimental models. Materials and methods: preparations tested for physical chemical analysis were dilutions 1 lm to 10 lm of Euphorbia tirucalli L. prepared from the latex and the juice of the plant. To rule the seasonal characteristics of this plant, 2 different populations were used, one collected in June 2007 and the other in May 2008. Furthermore, the cytotoxic effect of Euphorbia tirucalli 5 lm was tested on human breast cancer cells (MCF7) through MTT assay. Some differences among the two collections were observed. However, any clear correlation could be observed between physical chemical properties and biological activity.

Author Biographies

Sheila Garcia, Faculty of Pharmacy, Federal University of Rio de Janeiro -UFRJ, Rio de Janeiro

Departamento de Medicamentos

Rafael Cardoso Harduim, Faculty of Pharmacy, Federal University of Rio de Janeiro -UFRJ, Rio de Janeiro

Departamento de Medicamentos

Fortune Homsanin, Faculty of Pharmacy, Federal University of Rio de Janeiro -UFRJ, Rio de Janeiro

Departamento de Medicamentos

Carlos Renato Zacharias, UNESP

Departamento de Física e Química

Ricardo Machado Kuster, Núcleo de Pesquisa em Produtos Naturais, UFRJ

NPPN

Carla Holandino Quaresma, Faculty of Pharmacy, Federal University of Rio de Janeiro -UFRJ, Rio de Janeiro

DSc; BC Homeopathy. Department of Medicines, Faculty of Pharmacy, UFRJ.

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Published

2010-06-25

Issue

Section

Basic Research (Biology)

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