Modulation of acute inflammatory response in murine cutaneous leishmaniasis after treatment with thymulin 5ch and antimonium crudum 30cH

Authors

  • Fabiana Rodrigues Santana
  • Elisabeth Cristina Perez Hurtado
  • Marcia Dalastra Laurenti
  • Cidéli de Paula Coelho
  • Thayna Neves Cardoso
  • Lika Osugui
  • Nilson Roberti Benites
  • Leoni Villano Bonamin

Abstract

Leishmaniasis is a zoonosis caused by intracellular protozoa of the mononuclear phagocyte system; the modulation of these cells may impact in the host/parasite relationship. The present study evaluated the action of homeopathic Thymulin 5 cH and Antimonium crudum 30 cH in the experimental infection of Leishmania (L.) amazonensis. Balb/c mice were inoculated with 2x106 promastigotes of Leishmania (L.) amazonensis into the footpad and, after 48 hours, different cell populations such as CD19+ (lymphocytes B1 and B2), CD5+ (B1subtype), CD11b+ (phagocytes), CD4+ and CD8+ (T lymphocytes) were analyzed in the peritoneal washing fluid by flow cytometry. Histomorphometry of the paw, lymph nodes and spleen were also made after Giemsa staining and immunohistochemistry for CD3, CD11b and CD45RA (T lymphocytes, B lymphocytes and macrophages, respectively). The results showed increase in B-lymphocytes (X2, p=0.0001), in particular, B2 cells (X2, p=0.0001) in the peritoneum, in the group treated with Antimonium crudum 30 cH. There was no difference in the number of amastigote forms of Leishmania (L.) amazonensis in the lesion. There were significant differences for CD45RA+ lymphocyte area in the lymph node, comparing the total area (p= 0.0001), Mantle Zone (p=0.0010) and paracortical area (p=0.0003). The spleen also showed increase of CD45RA+ cells, defined by scores (p=0.0001), in the groups treated with thymulin 5 cH and Antimonium crudum 30 cH, when compared to the control. CD11b+ cells were also increased in these groups (p=0.0001). We conclude that the homeopathic medicines interfered in the inflammatory and immune responses in the murine experimental infection by Leishmania (L.) amazonensis.

Downloads

Published

2013-08-26

How to Cite

Santana, F. R., Hurtado, E. C. P., Laurenti, M. D., Coelho, C. de P., Cardoso, T. N., Osugui, L., Benites, N. R., & Bonamin, L. V. (2013). Modulation of acute inflammatory response in murine cutaneous leishmaniasis after treatment with thymulin 5ch and antimonium crudum 30cH. International Journal of High Dilution Research - ISSN 1982-6206, 12(44), 151–151. Retrieved from https://highdilution.org/index.php/ijhdr/article/view/665

Most read articles by the same author(s)

1 2 3 4 5 6 7 > >>