International Journal of High Dilution Research - ISSN 1982-6206 <div style="width: 100%; text-align: justify; font-size: 12px;">IJHDR is the first full free open-access electronic journal specializing in the multidisciplinary field of High Dilution (HD) research. Hosted by GIRI and supported by HRI (, it is peer-reviewed, with an international editorial board and published quarterly. Indexed at LILACS/BIREME/PAHO/WHO and EBSCO, and inserted at SCOPUS, Qualis-CAPES, Latindex, OJS/PKP, Google Scholar, DOAJ, NSDL/NSF, Open J-Gate, JournalSeek, and BVS.</div> Groupe International de Recherche sur l'Infinitesimal - GIRI en-US International Journal of High Dilution Research - ISSN 1982-6206 1982-6206 <p>&lt;a rel="license" href=""&gt;&lt;img alt="Creative Commons License" style="border-width:0" src="" /&gt;&lt;/a&gt;&lt;br /&gt;This work is licensed under a &lt;a rel="license" href=""&gt;Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License&lt;/a&gt;.</p> <!-- <p>Upon acceptance of an article for publication, author(s) recognize Groupe International de Recherche sur l'Infinitésimale (GIRI) as the <strong>Licensor</strong>. International Journal of High Dilution Research (IJHDR) has adopted Creative Commons Public License (CCPL) as CC-by-nc-nd.</p><a href="" rel="license"><img style="border-width:0" src="" alt="Creative Commons License" align="absMiddle" /></a> Licensed to <a href="" rel="GIRI">GIRI </a>under <a href="" rel="license">CCPL</a>. <p>Under this license, others are allowed to download your article from IJHDR website and share it with others as long as they mention authors and licensor as well as link back to IJHDR, but they cannot change it any way nor can use it for commercial purposes.</p><p>More information at: <a href=""></a></p> --> Editorial Francisco Eizayaga Copyright (c) 2020 Francisco Eizayaga 2021-03-03 2021-03-03 19 4 01 01 10.51910/ijhdr.v19i4.1070 Nanoparticle characterization of HOMOEO AGROCARE (Agro Homeopathic Drug) by HRTEM and EDS analysis Background: Use of Homeopathy as a therapeutic tool is still very nascent in plant science and agriculture. Therapies used for plant growth and diseases are not relevant for plants alone, but they also drastically influence the ecosystem of the organic world too. Use of pesticides and chemical fertilizers was a boon during the initial phase of green revolution around the world. Unfortunately, later they became the major reason of chronic illnesses like cancers and a cause for soil degradation all over. Current scenario demands the use of alternative models in agricultural practices in-order to prevent diseases and to maintain the health status of the population. This is also very important to get rid of the damages done to the soil. Aims: Present study aimed at analyzing the material content of the agro homeopathic drug, ‘Homeo agrocare’. Methdology: High resolution transmission electron microscope (HRTEM) and Energy dispersion spectroscopy (EDS) was used to evaluate the material content of the drug. Results: (1) the drug solution contains plenty of nanoparticles (NPs). (2) the size of NPs ranges between 4.99nm - 120nm. (3) Twenty elements were identified in the fields studied. Conclusion: Study conclusively proved the presence of NPs of the original drug materials used in the ‘Homoeo agrocare’ drug solution. The presence of NPs of the original drug substances suggest that the drug action of ‘Homoeo agrocare’ in plants is due to the epigenetic modifications initiated by the NPs in the plant genetics. Rajendran Scaria Edacheril Copyright (c) 2020 Rajendran Scaria Edacheril 2021-03-03 2021-03-03 19 4 10 22 10.51910/ijhdr.v19i4.1025 Book Review of COVID -19: Introductory Handbook in Homoeopathy Not applicable as this is a Book Review Pritha Mehra Copyright (c) 2020 PRITHA MEHRA 2021-03-03 2021-03-03 19 4 23 24 10.51910/ijhdr.v19i4.1063 Homoeopathic Viscum album (10-3) is More Cytotoxic to in vitro Culture of Human Breast Cancer Cell Line than to Human Mesenchymal Stem Cells BACKGROUND Adenocarcinomas can be of several types, and MCF-7 is an adenocarcinoma of human breast cell line useful as preclinical model to screen therapeutic agents such as ultra-diluted Viscum album, an European plant whose extract is commonly used in cancer therapy. AIMS MCF-7 and mesenchymal stem cells were used to evaluate the in vitro cytotoxicity of homoeopathic Viscum album 1x10-3 (VAD3). METHODS cells were cultured for 24 hours in controlled environment (37.5oC and 5% CO2) in 96-well plates. After this time, VAD3 was added to the culture medium in concentrations varying from 10 to 100 ?L/mL for MTT assay (evaluation of viability of cells). A control group was maintained with culture medium only. After 48 hours, the procedures of analysis of cells viability were performed. RESULTS MTT assay showed that the concentrations of 42 ?L/mL and 62 ?L/mL were able to reduce cell viability to 50% in MCF-7 and mesenchymal stem cells, respectively, which means that half of the cells cultured were dead after 48 hours in contact with VAD3. CONCLUSION Viscum album presented higher cytotoxic action on human breast cancer cell line culture than on mesenchymal stem cells. This medicine is extensively used against cancer, and the use of the homoeopathic form of it brings new possibilities as no or fewer adverse effects would be present. Ana Catarina Viana Valle Lana Ribeiro Aguiar Hilana dos Santos Sena Brunel Patricia Furtado Malard Carla Lujan Pereira Villarroel Rosângela Vieira Andrade Copyright (c) 2020 Ana Catarina Viana Valle, Lana Ribeiro Aguiar, Hilana dos Santos Sena Brunel, Patricia Furtado Malard, Carla Lujan Pereira Villarroel, Rosângela Vieira Andrade, Dr. 2021-03-03 2021-03-03 19 4 25 34 10.51910/ijhdr.v19i4.1036 Preparation of Coronavirus nosodes sourced from a clinical sample of SARS-Cov-2 positive patient, inactivated strain, and spike glycoprotein Introduction Nosodes, the homeopathic preparations sourced from biological materials including clinical samples, cultures of organisms, and diseased tissues have been in use against the source-specific infections as well as other diseases. The nosodes have demonstrated some efficacy in managing epidemics, such as influenza, dengue, and leptospirosis. This article presents the need and process of development of nosodes from the SARS-CoV-2 to explore its prophylactic and therapeutic potentials against certain related viral diseases. MATERIALS AND METHODS A clinical sample of SARS-Cov-2 positive patient, based on the cycle threshold (CT) value of the qRT-PCR, heat-inactivated SARS-CoV-2, and spike glycoprotein all were processed for making nosodes as per the method described in Homoeopathy Pharmacopoeia of India. Molecular tests, such as qRT- PCR and sterility tests were performed to establish the live organisms, RNA material, and the absence of contamination. RESULT Three variants of Coronavirus Nosode were developed using a clinical sample, heat-inactivated SARS-CoV-2, and spike glycoprotein. In potencies 3c and above, no detectable SARS-CoV-2 RNA material was found by PCR. The analytical results for nosodes were reported as compliant for sterility testing as per the IP. CONCLUSION Three variants of Coronavirus nosodes were prepared which need to be evaluated further through pre-clinical and clinical studies. Sandeepan Mukherjee Gitanjali Talele Abhay Chowdhary Shashikant Vaidya Rajesh Deshmukh Rakesh Rawal Rajesh Shah Copyright (c) 2020 Sandeepan Mukherjee, Gitanjali Talele, Abhay Chowdhary, Shashikant Vaidya, Rajesh Deshmukh, Rakesh Rawal, Rajesh Shah 2021-03-03 2021-03-03 19 4 02 09 10.51910/ijhdr.v19i4.1054